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BIOTECHNOLOGY A LABORATORY SKILLS COURSE TEACHER SUPPLEMENT2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载

J.KIRK BROWN 著
出版社： BIO-RAD
ISBN：9780983239611
出版时间：2011
标注页数：188页
文件大小：77MB
文件页数：194页
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图书目录

Chapter 1 Implementation Guide2

How to Use This Book2

Teaching Strategies4

Assessment6

How to Start a Biotechnology Course7

Independent Research Projects10

Chapter 2 Laboratory Skills12

Activity 2.1 DNA Extraction and Precipitation12

Activity 2.2 Pipetting16

Activity 2.3 Kool-Aid Column Chromatography19

Activity 2.4 Making Solutions23

Activity 2.5 Titration28

Activity 2.6 Writing a Standard Operating Procedure31

Chapter 3 Microbiology and Cell Culture34

Activity 3.1 Making Microbiology Media34

Activity 3.2 Disk Diffusion Test （Modified Kirby-Bauer Test）40

Activity 3.3 Microbes and Health: An Illustration ofKoch's Postulates44

Activity 3.4 Gram Staining49

Activity 3.5 Quantifying Bacterial Numbers52

Activity 3.6 Staining Eukaryotic Cells55

Chapter 4 DNA Structure and Analysis58

Activity 4.1 Restriction Site Prediction Using NEBcutter58

Activity 4.2 Casting Agarose Gels61

Activity 4.3 Dye Electrophoresis65

Activity 4.4 Restriction Digestion and Analysis of Lambda DNA69

Activity 4.5 Forensic DNA Fingerprinting75

Activity 4.6 Plasmid Mapping80

Chapter 5 Bacterial Transformation & Plasmid PurificationActivity 5.1 Bacterial Transformation with S3 Plasmid84

Activity 5.2 Bacterial Transformation with pGLO Plasmid89

Activity 5.3 Purification of S3 and pGLO Plasmids93

Activity 5.4 DNA Quantitation97

Chapter 6 The Polymerase Chain Reaction102

Activity 6.1 STR PCR Analysis102

Activity 6.2 GMO Detection by PCR107

Activity 6.3 Detection of the Human PV92 Alu Insertion114

Chapter 7 Protein Structure and Analysis120

Activity 7.1 Protein Quantitation Using the Bradford Assay120

Activity 7.2 Size Exclusion Chromatography124

Activity 7.3 GFP Purification by Hydrophobic Interaction Chromatography127

Activity 7.4 SDS-PAGE of Fish Muscle132

Activity 7.5 Biofuel Enzyme Assay137

Activity 7.6 Exploring Bioinformatics with GFP143

Chapter 8 Immunological Applications148

Activity 8.1 Ouchterlony Double Immunodiffusion Assay148

Activity 8.2 Serum Antibody Detection by ELISA152

Activity 8.3 Quantitative ELISA156

Activity 8.4 Western Blotting160

Appendices167

Appendix Ⅰ: Debate Activity167

Appendix Ⅱ: Technical Writing Rubric169

Appendix Ⅲ: Semilog Graph Paper170

Appendix Ⅳ: Activities Sorted By Skill Level171

Appendix Ⅴ: Pacing Guides172

Appendix Ⅵ: Purchasing Guide174

Appendix Ⅶ: Trademarks and Legal Notices187